EZQuant–ELN Knowledge Base

MOPS (x10)	Table of Contents

MOPS (x10) - 1 liter

• Add 41.85g 4-morpholinopropanesulfonic acid (MOPS – free acid, Mw=209.27) and 6.8g sodium acetate-3H₂O (NaOAc-3H₂O, Mw=136.08) to 800ml DEPC-treated ddH₂O. Stir until completely dissolved.
• Add 20ml of DEPC-treated 0.5M Na₂EDTA solution and adjust pH to 7.0 with 10M NaOH.
• Adjust volume to 1 liter with DEPC-treated ddH₂O.
•  Divide into aliquots of 200ml
• Store at 4 ^oC, protect from light.  
• If the solution turns yellow, use a new aliquot.