EZQuant–ELN Knowledge Base

TBS (x10)	Table of Contents

TBS (x10) - 1 liter (Tris Buffered Saline)

• Dissolve 60.6g Tris(hydroxymethyl)aminomethane (Tris, Mw=121.14)  and
  88g sodium chloride (NaCl, Mw=58.44)  in 800ml ddH₂O.
• Adjust pH to 7.4 with HCl.
• Adjust volume to 1 liter with ddH₂O.
• Sterilize by autoclaving or filtration.
• Store at room temperature.