EZQuant–ELN Knowledge Base

Na-Phosphate buffer (x1)	Table of Contents

• Prepare 0.2M sodium phosphate, mono-sodium salt:
  dissolve 27.6g NaH₂PO₄-H₂O (Mw=138) in 500ml ddH₂O and adjust volume to 1 liter with ddH₂O.
• Prepare 0.2M sodium phosphate, di-sodium salt:
  dissolve 53.62g Na₂HPO₄-7H₂O (Mw=268.1) in 500ml ddH₂O and adjust volume to 1 liter with ddH₂O.

  Mix the two sodium phosphate solutions with 100ml H₂O as specified in the table below in order to obtain a 200ml solution of 0.1M Na-Phosphate with a specific pH. (Note: it is strongly recommended to check the final pH value with a pH meter).    

   

   

0.1M Na-Phosphate buffer (x1) - 200ml